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3.5 Quantification of thiols, disulfides, and GSH in cell lysate supernatant and cell lysate pellet suspension of OVCAR-3 cells Quantification of thiols by HPLC was based on the peak area ratio of TNB to the internal standard (I.S.) and determined by reference to a TNB standard curve expressed as GSH equivalents

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An intriguing possibility is that, with the advent of multicellularity and the generation of specialized cell types, new tissue-restricted proteins (e.g., HOX) evolved within the genetic toolkit of animals to harness the function of these ancient activators in a cell type- or tissue-specific manner