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Full article: tRNA synthase suppression activates de novo cysteine synthesis to compensate for cystine and glutathione deprivation during ferroptosis
Lipid peroxidation assay protocol summary: – add TBA reply to samples and necessities, incubate at 95ºC for 60 min, cool in ice bathtub for 10 min – swap to wells of microplate – analyze with microplate reader For higher sensitivity, precipitate with n-butanol, centrifuge, dry and resuspend pellet sooner than analysis
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Novel studies have addressed riboflavin-dependent cellular processes in various diseases (Reference Thakur, Tomar and Singh11)
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