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Targeted demethylation of specific loci successfully restored sperm count by 80%, confirming that DNA methylation changes represent functional rather than merely correlative alterations ( 5.4.2 Single-cell epigenomic profiling Advanced single-cell technologies including scRNA-seq and ATAC-seq have revealed persistent chromatin accessibility changes in F1-F3 germ cells, providing unprecedented resolution of transgenerational epigenetic inheritance mechanisms (119)
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